Ready-to-Assay 5-HT₆ Serotonin Family Receptor Frozen Cells

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Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The neurotransmitter serotonin/5-hydroxytryptamine (5-HT) regulates a wide variety of neurological functions. A family of 13 receptors (12 GPCRs and one ion channel) mediates the effects of serotonin. The serotonin receptor 5-HT6 is a Gs coupled receptor expressed solely in the CNS, primarily in the limbic and cortical regions. 5-HT6 appears to play a role in memory and learning, obesity, psychosis, anxiety and epilepsy (Woolley et al., 2004; Fisas et al., 2006). In particular, a 5-HT6 -selective agonist caused significant weight loss in a rat model of diet-induced obesity. Millipore′s cloned human 5-HT6 -expressing cell line is made in the Chem-10 host, which supports high levels of recombinant 5-HT6 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at 5-HT6.

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.