製品名
SIGMAFAST™ BCIP®/NBT, tablet
form
tablet
Quality Level
solubility
water: 10 mL, clear, yellow
storage temp.
−20°C
Application
SIGMAFAST BCIP®/NBTは、以下の基質として使用されています:
- イムノブロッティングにおける抗マウスIgG結合アルカリホスファターゼ
- 初代骨芽細胞の組織化学的染色におけるアルカリホスファターゼ
- 骨髄間質細胞におけるアルカリホスファターゼ染色
Preparation Note
1錠を10 mLの脱イオン水に溶解すると、即使用可能なBCIP/NBT含有バッファ-溶液(pH 9.5)が得られます。
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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保管分類
11 - Combustible Solids
プロトコル
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
パラフィン包埋組織切片中の特定の抗原を染色および可視化する免疫組織染色(IHC)の全プロトコルをご紹介します。
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
資料
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
Aneela Yasmeen et al.
Molecular biotechnology, 58(12), 807-820 (2016-10-21)
The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct