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Merck

04-1525

Anti-TAFII250 Antibody, clone 6B3

clone 6B3, from mouse

別名:

Cell cycle gene 1 protein, TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa, TAF1 RNA polymerase II, TATA box binding protein-associated factor, 250kDa, TATA box binding protein (TBP)-associated factor, RNA polymerase II,

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
6B3, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
-
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biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6B3, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TAF1(6872)

General description

Calculated molecular weight is 212 kDa However protein is usually observed at 250 kDa band.
TATA-binding protein associated factor 250 (TAFII250) is the largest subunit of the transcription factor TFIID. Studies have shown that TAFII250 has serine/threonine domains that can autophosphorylate and transphosphorylate the large subunit of TFIIF. More recent studies have shown that TAFII250 is also a histone acetyltransferase, suggesting that transcriptional co-activators may also have a role in targeting acetylation activity to promoters.

Immunogen

Epitope: Unknown
Human TAFII250

Application

Anti-TAFII250 Antibody, clone 6B3 is a Mouse Monoclonal Antibody for detection of TAFII250 also known as Cell cycle gene 1 protein & has been validated in WB, ICC, IHC & IP.
Immunoprecipitation
Cited by external research group using a representative lot. See Hilton, H.L., et. al (2005) Mol. Cell. Bio. 25(10):4321–4332.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Biochem/physiol Actions

This antibody recognizes TAFII250.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected TAFII250 on 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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保管分類

12 - Non Combustible Liquids

wgk

WGK 1



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