04-1578
Anti-Bruton′s tyrosine kinase (Btk) Antibody, clone 10D11
clone 10D11, from mouse
別名:
Agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase, Bruton agammaglobulinemia tyrosine kinase, Bruton tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, tyrosine-protein kinase BTK
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
10D11, monoclonal
Application:
IP, WB
Citations:
2
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10D11, monoclonal
species reactivity
rat, human
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BTK(695)
General description
Btk (Bruton’s tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. BTK plays a crucial role in B cell activation and development. Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner. Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA).
~77 kDa
Immunogen
Epitope: Intracellular
GST-tagged recombinant protein corres-ponding to human Btk.
Application
Detect Bruton′s tyrosine kinase (Btk) using this Anti-Bruton′s tyrosine kinase (Btk) Antibody, clone 10D11 validated for use in WB & IP.
Immunoprecipitation Analysis: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Btk in the presence of 0.5% SDS. Not recommended for IP/kinase assays.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunological Signaling
Immunological Signaling
Biochem/physiol Actions
Recognizes human Btk, MW ~77 kDa.
A higher molecular weight protein may be detected, which is thought to be a phosphorylated species of Btk or a related protein.
A higher molecular weight protein may be detected, which is thought to be a phosphorylated species of Btk or a related protein.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Raji cell lysate
Raji cell lysate
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 0.05- 1 µg/mL of this antibody detected Btk on 10 µg of Raji cell lysate.
Western Blot Analysis: 0.05- 1 µg/mL of this antibody detected Btk on 10 µg of Raji cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1