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Merck

05-1245

Anti-O-GlcNAc Antibody, clone 9D1.E4(10)

clone 9D1.E4(10), from mouse

別名:

O-GlcNAc transferase p110 subunit, O-GlcNAc transferase subunit p110, O-linked GlcNAc transferase, O-linked N-acetylglucosamine (GlcNAc) transferase

(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase, O-linked N-acetylglucosam

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
9D1.E4(10), monoclonal
Species reactivity:
human
Application:
WB
Citations:
6
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Quality Segment

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

9D1.E4(10), monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

glycosylation

Gene Information

human ... OGT(8473)

General description

O-linked N-acetylglucosamine (O-GlcNAc), is a member of the O-GlcNAc transferase family. O-GlcNAc is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine/threonine hydroxyl groups. It plays an important role in nutrient sensing, gene expression, and protein degradation. O-GlcNAc is widely expressed in the pancreas and is present in smaller amounts in skeletal muscle, heart, brain and placenta.
Various

Immunogen

SANM-conjugated linear peptide corresponding to human O-GlcNAc.

Application

Detect O-GlcNAc using this Anti-O-GlcNAc Antibody, clone 9D1.E4(10) validated for use in WB.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Biochem/physiol Actions

This antibody recognizes O-GlcNAc.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal antibody in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C for up to 12 months.

Analysis Note

Control
HEK293 cell lysate
Evaluated by Western Blot in O-GlcNAc transferease transfected HEK293 cell lysate and O-GlcNAcase transfected transfected HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected O-GlcNAc on 10 µg of
O-GlcNAc transferease transfected HEK293 cell lysate and O-GlcNAcase transfected transfected HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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保管分類

12 - Non Combustible Liquids

wgk

WGK 1



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