Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

~17 kDa observed

Epitope: Trimethylated Lys27

Linear peptide corresponding to human Histone H3 trimethylated at Lys27.

Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1 is a mouse monoclonal antibody for detection of trimethyl Histone H3 (Lys27) also known as H3K27me3, Histone H3 (tri methyl K27) & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of modified and nonmodified peptides.

Western Blot (Snap i.d.) Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of HeLa acid extract.

Immunocytochemistry Analysis: A 1:125 dilution from a representative lot detected Histone H3 in A431 and NIH/3T3 cells.

Dot Blot Analysis: Histone peptides with various modifications (see table) were transferred to
PVDF membrane and probed with Anti-Trimethyl Histone H3 (Lys4) (1:100 dilution). Proteins were visualized using a Donkey Anti-rabbit IgG conjugated to HRP and a chemiluminescence detection systems.

This antibody recognizes Histone H3 trimethylated at Lys27.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa acid extract

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: A 1:50,000 dilution of this antibody detected Histone H3 in 10 µg of HeLa acid extract.

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