Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody

110-115kDa

KLH-conjugated, synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5

Peptide Inhibition Analysis:
HeLa cell lysate, HeLa cell lysate+ non-phosphorylated protein, and HeLa cell lysate + phosphorylated peptide were probed with Cat. No. 07-507, Anti-phospho-BMK 1/ERK5 (Thr218/Tyr220) (1µg/mL(1:1000) dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system.

Arrow indicates BMK1/ERK5 (~115 kDa) on HeLa cell lysate & HeLa cell lysate + non-phosphorylated peptide. No band is present on the HeLa cell lysate + phosphorylated peptide indicating successful peptide inhibition of the antibody.

Research Category
Signaling

Research Sub Category
MAP Kinases

This Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody is validated for use in WB for the detection of phospho-BMK1/Erk5 (Thr218/Tyr220).

Recognizes phospho-BMK1/Erk5 (Thr218, Tyr220).

Affinity purified immunoglobulin in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

ImmunoAffinity Purified

Maintain for 1 year at 2-8°C from date of shipment.

Evaluated by Western Blotting/Peptide Inhibition Analysis in HeLa cell lysate.

Western Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected phospho-BMK1/Erk5 (Thr218/Tyr220) in HeLa cell lysate.

Control
Sorbitol-treated HeLa cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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