Anti-trimethyl Histone H3 (Lys27), Alexa Fluor^™ 647 Conjugate Antibody

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 07-449.

~17 kDa observed (Refer to Cat. No. 07-449)

Linear peptide corresponding to Histone H3 trimethylated at Lys27.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-trimethyl Histone H3 (Lys27) antibody is validated for use in ICC for the detection of trimethyl Histone H3 (Lys27).

Protein A purified

Purified rabbit conjugate antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry Analysis: in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys27) in HeLa cells.

Alexa Fluor^™ is a registered trademark of Life Technologies.

ALEXA FLUOR is a trademark of Life Technologies

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