AB10518
Anti-DARPP-32 Antibody
from rabbit, purified by affinity chromatography
別名:
protein phosphatase 1, regulatory (inhibitor) subunit 1B, dopamine and cAMP-regulated neuronal phosphoprotein 32, dopamine and cAMP regulated phosphoprotein, protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotei
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
-
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, rhesus macaque
species reactivity (predicted by homology)
canine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Ppp1R1B(19049)
General description
Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family. DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation. It possesses at least four sites that can be phosphorylated. Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor. However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation. This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors. Studies suggest that it may also have a role in the Circadian System photic pathway. Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues.
~ 32 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to mouse DARPP-32 near the N-terminus.
Application
Anti-DARPP-32 Antibody is an antibody against DARPP-32 for use in WB & IP.
Research Sub Category
Neurotransmitters & Receptors
Ion Channels & Transporters
Neurotransmitters & Receptors
Ion Channels & Transporters
Western Blot (Snap i.d.) Analysis: 1 µg/mL of this antibody from a previous lot detected DARPP-32 on 10 µg of rat brain tissue lysate.
Immunoprecipitaion Analysis: 10 µg of this antibody from a previous lot immunoprecipitated DARPP-32 from rat brain RIPA lysate.
Immunoprecipitaion Analysis: 10 µg of this antibody from a previous lot immunoprecipitated DARPP-32 from rat brain RIPA lysate.
Biochem/physiol Actions
This antibody recognizes DARPP-32 near the N-terminus.
Analysis Note
Evaluated by Western Blot on rat brain tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected DARPP-32 on 10 µg of rat brain tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected DARPP-32 on 10 µg of rat brain tissue lysate.
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保管分類
12 - Non Combustible Liquids
WGK
WGK 1