Anti-Calnexin Antibody

Calnexin is a Ca++ binding protein found in ER transmembranes that binds to specific n-linked oligosaccharides found on newly synthesized proteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRμ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.

~70 kDa observed

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human Calnexin.

Anti-Calnexin Antibody is an antibody against Calnexin for use in WB, IC, IH(P).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Calnexin in NIH/3T3 cells.

Immunohistochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected Calnexin in human brain and human prostate tissues.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This antibody recognizes Calnexin at the C-terminus.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human brain tissue lysate

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: A 1:10,000 dilution of this antibody detected Calnexin on 10 µg of human brain tissue lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.