Anti-Tau phospho Serine 409 Antibody

Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer′s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Serine 409 is phosphorylated by PKA in vitro and has shown to be phosphorylated in AD brain.

Synthetic peptide of amino acids surrounding the phosphoSerine 409 site of human Tau.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Tau phospho Serine 409 Antibody is validated for use in WB for the detection of Tau phospho Serine 409.

Western blot: 1:1,000. Suggested blocking buffer is 5% BSA-TBST for one hour at room temperature. Suggested antibody dilution buffer is 1% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature.

Optimal working dilutions must be determined by the end user.

Tau phosphoSerine 409. The antibody recognizes Tau pSerine 409 in samples of recombinant human Tau treated with PKA for 45 minutes. The reactivity of the antibody is blocked with the pSerine 409 peptide but not the non-phosphopeptide or a generic phosphoSerine-containing peptide.

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

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