Anti-TNRC6B Antibody

TNRC6B (trinucleotide repeat containing 6B) is a argonaute-associated protein that localizes to cytoplasmic P bodies and is essential for control of miRNA-guided mRNA cleavage. TNRC6B is one of the mammalian homologs of the metazoan GW182 and is required for well-organized miRNA repression. Studies suggest that TNRC6B-mediated miRNA silencing is promoted by the C-terminal silencing domain.

~150/190 kDa observed. (Meister, G., et al. (2005). Current Biology. 15:2149–2155). An uncharacterized band at ~39 kDa may be observed in some lysates.

Epitope: N-terminan domain of TNRC6B

KLH-conjugated linear peptide corresponding to human TNRC6B.

Anti-TNRC6B Antibody is a Rabbit Polyclonal Antibody for detection of TNRC6B also known as Trinucleotide repeat-containing gene 6B protein & has been validated in WB, ICC & IP.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected TNRC6B in HeLa cells. (Zipprich, J., et al. (2009). RNA. 15:781–793.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Antibody recognizes TNRC6B

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected TNRC6B on 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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