Anti-TopBP1 Antibody

Recombinant protein corresponding to human TopBP1.

~180 kDa observed

Western Blotting Analysis: A representative lot detected TopBP1 in U2OS and Saos-2 (Jeon, Y., et al. (2007). J. Biol. Chem. 282(20):14882-14890).
Western Blotting Analysis: A representative lot detected TopBP1 in mouse embryonic stem cells, early mouse embryos, and 3T3 cells (Jeon, Y., et al. (2011). J. Biol. Chem. 286(7):5414-5422).
Immunocytochemistry Analysis: A representative lot detected TopBP1 in 3T3, MEF, HS68, and IMR90 cells (Jeon, Y., et al. (2011). J. Biol. Chem. 286(7):5414-5422).

Rabbit polyclonal serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in Saos-2 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected TopBP1 in 10 µg of Saos-2 cell lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.