Anti-Aprataxin Antibody

Aprataxin (UniProt Q7Z2E3; also known as FHA-HIT, Forkhead-associated domain histidine triad-like protein) is encoded by the APTX (also known as AOA, AOA1, AXA1, EAOH, EOAHA) gene (Gene ID 54840) in human. Aprataxin contains an N-terminal forkhead-associated (FHA; a.a. 38-87) domain akin to that found in the DNA end damage repair factor polynucleotide kinase phosphatase (PNKP), followed by a histidine triad (HIT; a.a. 182-287) domain found in nucleoside hydrolases and transferases, and a C-terminal C2H2 type Zinc finger domain. Aprataxin exhibits both DNA- and RNA-binding activity and plays a role in reversing toxic 5′-adenylated (5′-AMP) DNA lesions generated by DNA ligases when encountering nicked DNA structures with abnormal DNA termini. Aprataxin functions as a DNA ligase proofreader to directly reverse damaged 5′-adenylated termini of DNA strand breaks that have been subjected to DNA damage-induced abortive processing by DNA ligases. Ataxia with oculomotor apraxia type 1 (AOA1) is an autosomal recessive neurological syndrome caused by an impairment in DNA repair as a result of APTX gene mutations. The related type 2 disorder AOA2 is caused by a Senataxin-deficiency due to SETX gene mutations.

~43 kDa observed.

Recombinant full-length human aprataxin.

Anti-Aprataxin Antibody is an antibody against Aprataxin for use in Western Blotting, Immunoprecipitation.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blotting Analysis: A representative lot detected wild-type and the V263G mutant form of recombinant human aprataxin (Kijas, A.W., et al. (2006). J. Biol. Chem. 281(20):13939-13948).
Western Blotting Analysis: A representative lot detected aprataxin in lysates from HeLa and control lymphoblastoid cell lines (C3ABR and C2ABR), but not from AOA1 cell lines (L939, L938) derived from patients with the neurological disorder ataxia with oculomotor apraxia type 1 (AOA1) (Becherel, O.J., et al. (2006). Hum. Mol. Genet. 15(14):2239-2249).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated nucleolar proteins nucleolin and nucleophosmin with aprataxin from C3ABR lymphoblastoid cell lysate (Becherel, O.J., et al. (2006). Hum. Mol. Genet. 15(14):2239-2249).

Expected to react with all 13 human aprataxin spliced isoforms reported by UniProt (Q7Z2E3-1 through Q7Z2E3-13).

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aprataxin in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

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