Anti-PDE9A Antibody

PDE9A is a ubiquitous protein belonging to the cyclic nucleotide phosphodiesterase family which regulate the signaling of cyclic nucleotides such as cGMP and cAMP. PDE9A has a higher affinity for cGMP. It contains a catalytic site that associates with cofactors and hydrolyzes cGMP thereby terminating cGMP signaling for a number of cellular processes. PDE9A has been indirectly linked to signaling pathways involved in cognitive processes. Previous studies suggested that inhibition of PDE9A enhanced long-term potentiation (a widely studied mechanism of learning and memory); PDE9A may also effect changes in the plasticity of hippocampal neurons which are also important for certain types of cognitive processes. In addition to the brain, PDE9A is also highly expressed in the spleen, prostate, colon, kidney, and heart and may be involved in a range of physiological processes in these systems, via cGMP-amplified signaling.

~75 kDa observed. Uniprot describes 16 isoforms produced by alternative splicing, which have been observed at various molecular weights. (Kruse, L.S., et al. (2009). Brain Res. 1281: 25-34; Rentero, C., et al. (2003). Biochem Biophys Res Commun. 301(3):686-692.)

KLH-conjugated linear peptide corresponding to human PDE9A.

Detect PDE9A using this Anti-PDE9A Antibody validated for use in Western Blotting, IHC(P).

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Western Blot Analysis: A representative lot of this antibody detected PDE9A in human liver tissue lysate, and in mouse spleen and liver tissue lysates.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected PDE9 in human prostate tissue, and in possible golgi type II and basket cells in the Purkinje and molecular layers of mouse cerebellum.

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human spleen tissue lysate

Evaluated by Western Blot in human spleen tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDE9A in 10 µg of human spleen tissue lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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