Anti-NeuN Antibody

RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. The NeuN protein is localized in nuclei and perinuclear cytoplasm of most neurons in the mammalian central nervous system. It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord. However, it is not detected in Cajal-Retzius cells in the neocortex, Purkinje cells, and photoreceptor cells. NeuN expression has been reported as early as embryonic day 9.5 (E9.5) and by E12.5 it can be found in the developing ventral horns. It is also detected in the developing dorsal horns and in the dorsal root ganglion. Its expression is enhanced by retinoic acid treatment. It can serve as a pre-mRNA alternative splicing regulator and regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay. Initial characterization of NeuN was derived from usage of a monoclonal antibody (A60), but later identified as RBFOX3. Subsequent studies have shown that anti-NeuN antibodies can identify most types of neurons in the whole nervous system with some rare exceptions. NeuN knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume. They exhibit increased susceptibility to seizures and reduced anxiety-related behaviors compared with their wild-type littermates. Antibodies for NeuN predominantly associate with cell nuclei and, to a lesser extent, with the perinuclear cytoplasm. Two isoforms of the NeuN protein (46 and 48 kDa) are present in both locations but differ in their relative concentration in the nucleus and cytoplasm. (Ref.: Lin, YS et al. (2018). PLOS One. 13(2); e0192355; Mao, S., et al. (2016). Front. Neuroanat. 13;10:54).

Target molecular weight ~47 and 52 kDa observed; 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Anti-Neun, chicken polyclonal antibody detects Neun (RNA binding protein fox-1 homolog). It targets an epitope within 97 amino acids from the N-terminal.

Tested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected NeuN in rat E18 cortical cells.

Immunohistochemistry (Paraffin) Analysis: A 1:3,000 dilution from a representative lot detected NeuN in mouse brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Other homologies: Human (94% sequence homology).

This antibody recognizes the N-terminus of NeuN.

Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity purified

Recommended storage: +2°C to +8°C.

Evaluated by Western Blotting in mouse E16 brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NeuN in mouse E16 brain tissue lysate.

Control
Mouse brain E16 tissue lysate

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