ABT1340
Anti-Myosin-10/MYH10/Myosin IIB Antibody
from rabbit, purified by affinity chromatography
別名:
Myosin-10, Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain Iib, MHC IIB
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IF, WB
Citations:
2
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MYH10(4628)
General description
Myosin-10 (UniProt P35580; also known as Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIb) is encoded by the MYH10 (also known as NMMHCB) gene (Gene ID 4628) in human. Nonmuscle myosin II plays an important role in cell migration and invasion. The myosin II holoenzyme consists of two myosin heavy chains (MHCs), two essential light chains, and two regulatory light chains. Three different nonmuscle MHC II proteins exist in mammals, IIA (MYH9), IIB (MYH10), and IIC (MYH14), with both unique and overlapping roles. One of the initial steps in cancer cell metastasis is thought to be the process of epithelial–mesenchymal transition (EMT). Myosin IIB expression and myosin IIA heavy chain phosphorylation are upregulated upon TGF-β–induced EMT in mammary epithelial cells, while inhibition of myosin IIB expression in post-EMT mesenchymal cells reduces transmigration and invasion.
~250 kDa observed. 229.0 kDa (isoform 1), 230.8 kDa (isoform 2), 231.4 kDa (isoform 3), 232.5 kDa (isoform 4), 230.0 kDa (isoform 5) calculated. Uncharacterized band(s) may appear in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminal sequence of human myosin-10.
Application
Anti-Myosin-10/MYH10/Myosin IIB Antibody is an antibody against Myosin-10/MYH10/Myosin IIB for use in Western Blotting, Immunocytochemistry, Immunofluorescence.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Myosin-10/MYH10/Myosin IIB in 10 µg of MCF-10A cell lysate.
Western Blotting Analysis: A representative lot detected elevated MHC IIB expression in MDA-MB-468, MDA-MB-231, and MCF10A, while very low MHC IIB expression was detected in MCF7, T47D, BT-474, and SKBR3 (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Western Blotting Analysis: A representative lot detected MHC IIB levels in NMuMG murine mammary gland cells upon TGF-beta stimulation or shRNA-mediated hnRNP E1 knockdown. E1 overexpression obolished TGF-beta stimulated MHC IIB upregulation in NMuMG cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunocytochemistry Analysis: A representative lot detected MHC IIB immunoreactivity localized to the rear and perinuclear regions of migrating and transmigrating hnRNP E1-knockdown murine mammary gland E1-shRNA NMuMG cells by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% TritonX-100-permeablized cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunofluorescence Analysis: A representative lot detected MHC IIB immunoreactivity predominantly in the SAM-expressing myoepithelial layer, but not the cytokeratin 8-expressing luminal layer, in 4% paraformaldehyde-fixed, paraffin-embedded mouse mammary tissue sections by fluorescent immunohistochemstry (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Western Blotting Analysis: A representative lot detected elevated MHC IIB expression in MDA-MB-468, MDA-MB-231, and MCF10A, while very low MHC IIB expression was detected in MCF7, T47D, BT-474, and SKBR3 (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Western Blotting Analysis: A representative lot detected MHC IIB levels in NMuMG murine mammary gland cells upon TGF-beta stimulation or shRNA-mediated hnRNP E1 knockdown. E1 overexpression obolished TGF-beta stimulated MHC IIB upregulation in NMuMG cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunocytochemistry Analysis: A representative lot detected MHC IIB immunoreactivity localized to the rear and perinuclear regions of migrating and transmigrating hnRNP E1-knockdown murine mammary gland E1-shRNA NMuMG cells by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% TritonX-100-permeablized cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Immunofluorescence Analysis: A representative lot detected MHC IIB immunoreactivity predominantly in the SAM-expressing myoepithelial layer, but not the cytokeratin 8-expressing luminal layer, in 4% paraformaldehyde-fixed, paraffin-embedded mouse mammary tissue sections by fluorescent immunohistochemstry (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996).
Biochem/physiol Actions
Expected to react with all five spliced isoforms of human Myosin-10 reported by UniProt (P35580).
Analysis Note
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myosin-10/MYH10/Myosin IIB in 10 µg of A549 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myosin-10/MYH10/Myosin IIB in 10 µg of A549 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
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保管分類
12 - Non Combustible Liquids
WGK
WGK 1