CBL218
Anti-Keratin K3/K76 Antibody, clone AE5
clone AE5, Chemicon®, from mouse
別名:
Anti-HUMCYT2A, Anti-KRT2P
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
AE5, monoclonal
Application:
IHC (p), WB
Citations:
14
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
AE5, monoclonal
species reactivity
human, mouse, bovine, rabbit
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KRT3(3850)
General description
64 kDa
Immunogen
Rabbit corneal epithelial keratin
Application
Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).
Immunohistochemistry on frozen sections: 1:50.
Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).
Western blotting: 1:500.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).
Western blotting: 1:500.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
Physical form
Format: Purified
The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.
Preparation Note
For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 2
保管分類
12 - Non Combustible Liquids