DR1057
Anti-SQSTM1 Mouse mAb (2C11)
liquid, clone 2C11, Calbiochem®
別名:
Anti-Sequestosome 1
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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
2C11, monoclonal
Species reactivity:
human, mouse
Application:
—
Citations:
1
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
2C11, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SQSTM1(8878)
mouse ... Sqstm1(18412)
General description
Purified mouse monoclonal antibody. Recognizes the SQSTM1 protein.
Recognizes the SQSTM1 protein HeLa cells.
This Anti-SQSTM1 Mouse mAb (2C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of SQSTM1.
Immunogen
Full-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
NA
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells
HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Lee, H,K., et al. 2010. Immunity.32, 227.
Bjorkoy, G., et al. 2009. Methods Enzymol.452, 181.
Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet.18 4868.
Gal, J., et al. 2009. J. Neurochem.111 1062.
Mardakheh, F,K., et al. 2009.J. Cell Biol.187, 265.
Jung, H,S., et al. 2008. Cell Metab.8, 318.
Bjorkoy, G., et al. 2009. Methods Enzymol.452, 181.
Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet.18 4868.
Gal, J., et al. 2009. J. Neurochem.111 1062.
Mardakheh, F,K., et al. 2009.J. Cell Biol.187, 265.
Jung, H,S., et al. 2008. Cell Metab.8, 318.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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保管分類
12 - Non Combustible Liquids
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