Milli-Mark^® Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18

26 kDa Observed

The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl-2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Bcl-2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders. Bcl-2 phosphorylated at Ser-70 plays a critial role in the translocation of Bax from the cytosol to the mitochondria, and this may regulate NO-induced neuronal apoptosis. The molecular mechanism of Bcl-2 phosphorylation and its relationship to Bax is largely unknown.

Epitope: Ser70

KLH-conjugated linear peptide corresponding to human Bcl-2 phosphorylated at Ser70.

Detect phospho-Bcl-2 (Ser70) using this Milli-Mark Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in FC.

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

This antibody recognizes phosphorylated Bcl-2 at Ser70.

Protein A purified

Purified rabbit monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Jurkat cells.

Evaluated by flow cytometry using Jurkat cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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