Anti-Tryptase Antibody, Mast Cell, clone G3

31 kDa

Tryptase is the most abundant secretory granule-derived serine proteinase contained in mast cells. Tryptase has recently been used as a marker for mast cell activation and is involved with allergic response. Tryptase may act as a mitogen for fibroblast lines. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis.

Flow Cytometry Analysis: A previous lot of this antibody has been used on fixed cells in FACS using a conjugated secondary antibody. (Sperr, W., et al., 2001).

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

This Anti-Tryptase Antibody, Mast Cell, clone G3 is validated for use in FC, IH, WB for the detection of Tryptase.

Recognizes Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
(see product datasheet for specific buffer formulation)

Maintain at 2–8°C for the duration listed on the product datasheet. (See product datasheet for storage conditions)

Control
Mast cells, basophils

Human fetal skin lysate

Evaluated by western blot on human fetal skin lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected tryptase in 10 µg of human fetal skin lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.