Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free

Talin 1, also known as Talin 1, and encoded by the gene TLN1/KIAA1027, a large connecting protein located on the cytoplasmic side of most cellular projections. Talin appears to act as an anchoring protein connecting the cytoskeletal protein structures to the plasma membrane. Talin gets concentrated at regions of cell-substratum contact and in particular cells such as lymphocytes at regions of direct cell-cell contact. . Talin 1 binds with high affinity to vinculin and interacts with other proteins found in cellular projections including focal adhesion kinases (FAK) and intermediate filament proteins such as synemin and layilin. Additionally, Talin 1 because of its connections with cytoskeletal proteins talin is associated with many vital cell motion/growth processes from axon guidance to cell migration, muscle contraction, cell-cell interactions, immune responses and platelet activation to name but a few.

~270 kDa observed

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of human Talin 1.

Immunohistochemistry Analysis: A 1:2,000 and a 1:500 dilution from a representative lot detected Talin 1 in human prostate cancer sections and human lung tissue, respectively.

Immunohistochemistry Analysis: A representative lot detected Talin 1 in MeOH fixed fibroblasts.

Inhibition Analysis: A representative lot from an independent laboratory disrupts actin stress fibers and focal adhesions, and inhibits cell motility when microinjected into human fibroblasts (Bolton, S. J., et al. (1997). Cell Motil Cytoskeleton. 36(4): 363-376.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from shCTRL and shLRP-1-c1 carcinoma cell lysates (Dedieu, S., et al. (2008). Mol Cell Biol. 28(9):2980-2995.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from shCTRL and shLRP-1 cell lysates (Langlois, B., et al. (2010). PLoS One. 5(7):e11584.).

Immunoprecipitation/Western Blotting Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from PC3-2 cell lysates. Immunoprecipitated Talin 1 was then detected in Western Blotting (Trerotola, M. et al. (2012). J Cell Physiol. 227(11): 3670-3676.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Talin 1 in macrophages (Love, D. C., et al. (1998). Exp Parasitol. 88(3):161-171.).

Immunofluorescence Analysis: A representative lot from an independent laboratory determined that the level of disruption in talin-containing focal complexes is inhibited in LRP-1-silenced carcinoma cells (Dedieu, S., et al. (2008). Mol Cell Biol. 28(9):2980-2995.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Talin-1 in shCTRL and shLRP-1 transfected cells (Langlois, B., et al. (2010). PLoS One. 5(7):e11584.).

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free is validated for use in western blotting, IHC (Paraffin), IP, inhibition & immunofluorescence for the detection of Talin 1.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Talin 1 in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.