MAB3120
Anti-Cre Recombinase Antibody, clone 2D8
ascites fluid, clone 2D8, Chemicon®
別名:
Anti-Cre Antibody
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2D8, monoclonal
Application:
ELISA, ICC, IF, IHC, WB
Citations:
164
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2D8, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
Escherichia coli ... Cre(2777477)
General description
38 kDa
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. Anti-Cre Recombinase Antibody can be used to determine the success of gene manipulations in transgenic animals.
Immunogen
Cre recombinase (aa 77 to 343) fusion protein
Application
Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.
Immunofluorescence:
A previous lot of this antibody was used on IF.
Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.
Immunofluorescence:
A previous lot of this antibody was used on IF.
Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Cre recombinase. The epitope is located between aa 77 and 343.
Physical form
Ascites fluid. Contains no preservative
Unpurified
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
HeLa Nuclear Extract
HeLa Nuclear Extract
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
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