Anti-GFAP, clone GA5, AF^™ 647 Conjugate

50 kDa

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Purified Glial Fibrillary Acidic Protein (GFAP) from pig spinal cord (Debus, E., 1983).

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.

Immunocytochemistry:

5 μg/mL of a previous lot was used.

Immunohistochemistry:

5 µg/mL

Immunoblotting: Recognizes a 51kDa protein in reducing westerns of total brain lysates.

Optimal working dilutions must be determined by end user.

Research Category

Neuroscience

Research Sub Category

Neuronal & Glial Markers

Expected to cross-react with porcine, chicken, bovine, and rabbit.

The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.

Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.

DO NOT FREEZE.

Control

Mouse brain tissue, Astrocyte culture.

Routinely evaluated by Western Blot on Mouse brain lysate.

Western Blot Analysis:

1:1000 dilution of this lot detected Glial Fibrillary

Acidic Protein on 10 μg of Mouse Brain lysate.

Replaces: 04-1031; 04-1062

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.