Anti-Ezrin Antibody, clone 4A5, Ascites Free

Ezrin (UniProt Q8HZQ5; also known as Cytovillin, Villin-2, p81) is encoded by the EZR (also known as VIL2) gene (Gene ID 100008846) in Oryctolagus cuniculus (Rabbit) species. Ezrin, radixin, and moesin are the three ERM protein family members that crosslink actin filaments with plasma membranes and play an important role in the cell cortex architecture. In its inactive state, ezrin is located in the cytosol in a conformation involving intramolecular interaction between its N- and C-terminal. Ezrin is activated by phosphorylation, which causes a conformation change that disrupts the head-to-tail intramolecular interaction. Upon activation, ezrin binds to the cytoplasmic tails of multiple adhesion and signaling membrane molecules, such as CD44, growth factor receptors, and vascular adhesion molecule (VCAM1). The initiator methionine of ezrin is removed posttranslationally to yield the mature protein (a.a. 2-586) with an N-termina FERM domain (a.a. 2-295) that mediates interaction with microtubules.

~75 kDa observed. 69.41 kDa (Human/UniProt P15311 & Mouse/P26040), 69.39 kDa (Rat/UniProt P31977), and 69.22 kDa (Rabbit/Q8HZQ5) calculated. Uncharacterized band(s) may appear in some lysates.

Purified Ezrin from rabbit gastric parietal cells.

Anti-Ezrin Antibody, clone 4A5, Ascites Free is an antibody against Ezrin for use in Western Blotting, Immunohistochemistry, Immunoprecipitation, Electron Microscopy, Immunocytochemistry.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western Blotting Analysis: 0.5 µg/mL of this antibody from a representative lot detected Ezrin in 10 µg of HeLa cell lysate.
Immunohistochemistry Analysis: A representative lot detected a higher Ezin immunoreactivity in paraffin-embedded human medulloblastoma sections when compared with normal cerebellum sections (Haeberle, H., et al. (2012). Neoplasia. 14(7):666-669).
Immunohistochemistry Analysis: A representative lot immunostained parietal cells in rabbit gastric mucosa (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Ezin from human A431 cells. The immunoprecipitated Ezrin was phosphorylated on Ser66 from dbcAMP‐treated, but not cAMP‐treated, A431 cells (Wang, S., et al. (2009). Cell Res. 19(12):1350-1362).
Electron Microscopy Analysis: A representative lot detected Ezrin on microvilli of the intracellular canaliculi (IC) and the subapical region of resting parietal cells in Lowicryl HM20-embedded rabbit gastric gland ultrathin sections (Sawaguchi, A., et al. (2004). J. Histochem. Cytochem. 52(1): 77–86).
Immunocytochemistry Analysis: A representative lot detected exogenously expressed human GST fusion protein by fluorescent immunocytochemistry staining of formaldehyde-fixed, Triton X-100-permeabilized rabbit gastric parietal cells (Zhou, R., et al. (2003). J. Biol. Chem. 278(37):35651-35659).
Western Blotting Analysis: A representative lot detected both the endogenous rabbit Ezrin as well as exogenously expressed human Ezrin GST fusion proteins in transfected rabbit gastric parietal cells (Zhou, R., et al. (2003). J. Biol. Chem. 278(37):35651-35659).
ELISA Analysis: Clone 4A5 hybridoma culture supernatant was screened for its immunoreactivity by ELISA and found to react with both dentured and non-denatured 80 kDa Ezrin preparations from rabbit gastric glands/mucosa vesicular fractions (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).

Clone 4A5 reacted with both dentured and non-denatured Ezrin (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in L6 rat myoblast lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ezrin in 10 µg of L6 rat myoblast lysate.

Concentration: Please refer to lot specific datasheet.

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