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Merck

MAB4051

Anti-Angiotensin Converting Enzyme Antibody, clone 9B9

clone 9B9, Chemicon®, from mouse

別名:

ACE, CD143, DCP1, Peptidyl-dipeptidase A, Peptidase P

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9B9, monoclonal
Application:
ELISA, ICC, IHC, IP, WB
Citations:
7
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biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9B9, monoclonal

species reactivity

hamster, feline, human, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... AGT(183)

General description

The previously assigned protein identifier P22966 has been merged into P12821. Full details can be found on the UniProt database.

Immunogen

Angiotensin-converting enzyme from human lung

Application

Anti-Angiotensin Converting Enzyme Antibody, clone 9B9 detects level of Angiotensin Converting Enzyme & has been published & validated for use in ELISA, IP, WB, IC, IH.
Immunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.

Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).

Immunoblotting: 1:100: ~170-180kDa isoform, reducing conditions.

Immunoprecipitation: 1:1,000-1:10,000

ELISA : 1:1,000

Visualization of lung vessels (Danilov et al., 1989; Klibanov et al., 1988; Hiemisch et al., 1993)

Antibody mediated drug delivery into the lung (Danilov et al., 1991, 1994; Muzykantov et al., 1994, 1996)

Estimation of lung vessel injury (Muzykantov et al., 1991; Atochina et al., 1992)

Structure-function of ACE (Danilov et al., 1994)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Hormones & Receptors

Biochem/physiol Actions

Angiotensin-converting enzyme [ACE] (N-terminal), no reactivity to testicular/germinal form of human ACE (90kDa isoform)

Physical form

Format: Purified
Purified immunoglobulin. Liquid in PBS, pH 7.4, 150 mM NaCl containing 0.05% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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保管分類

12 - Non Combustible Liquids

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WGK 2



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