MAB8560
Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4
ascites fluid, clone 583-G8-G2-A4, Chemicon®
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
583-G8-G2-A4, monoclonal
Species reactivity:
human
Application:
IF
Citations:
2
biological source
mouse
Quality Segment
antibody form
ascites fluid
antibody product type
primary antibodies
clone
583-G8-G2-A4, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
isotype
IgG1
shipped in
wet ice
Application
Detect Poliovirus 1 using this Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4 validated for use in IF.
IFA at 1:100-1:400.
Optimal working dilutions must be determined by end user.
SUGGESTED IFA
PROTOCOL:
Substrate slides:
1. Scrape and spot cells onto microscope slide wells.
2. Air Dry.
3. Fix in cold acetone for 10 minutes at +4°C.
Stain:
1. Add 15 μL diluted polio antibody.
2. Incubate at +37°C for 30 minutes in a humid chamber.
3. Rinse in PBS for 5 minutes; repeat with fresh PBS.
4. Add 15 μL diluted labeled antibody.
5. Repeat steps 2 and 3
6. Mount with buffered glycerol.
Optimal working dilutions must be determined by end user.
SUGGESTED IFA
PROTOCOL:
Substrate slides:
1. Scrape and spot cells onto microscope slide wells.
2. Air Dry.
3. Fix in cold acetone for 10 minutes at +4°C.
Stain:
1. Add 15 μL diluted polio antibody.
2. Incubate at +37°C for 30 minutes in a humid chamber.
3. Rinse in PBS for 5 minutes; repeat with fresh PBS.
4. Add 15 μL diluted labeled antibody.
5. Repeat steps 2 and 3
6. Mount with buffered glycerol.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Biochem/physiol Actions
Reacts with the Poliovirus 1.
Preparation Note
Store at -20°C for up to 12 months in convenient aliquots. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable