Anti-phospho-RNAPII Antibody (Thr4), clone 1G7

RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

~220 kDa observed

Epitope: Phosphorylated Thr4

Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Thr4.

Detect the phospho-RNAPII (Thr4) protein using this Anti-phospho-RNAPII (Thr4), clone 1G7 validated for use in wb, IP, ICC & ChIP.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cell extracts (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cells (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Thr4) in stably transfected Raji cell lysates (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

This antibody recognizes RNAPII phosphorylated at Thr4.

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Untreated and lambda phosphatase treated HeLa cell lysates.

Evaluated by Western Blot in untreated and lambda phosphatase treated HeLa cell lysates.

Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-RNAPII (Thr4) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)

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