Anti-CASK Antibody, clone 2G5.1

Peripheral plasma membrane protein CASK (EC 2.7.11.1; UniProt O14936; also known as Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, hCASK, Protein lin-2 homolog, Trinucleotide repeat containing 8) is encoded by the CASK (also known as CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8) gene (Gene ID 8573) in human. CASK is a membrane-associated guanylate kinase (MAGUK) family member involved in the regulation of cytoskeletal remodeling and cell migration. It consists of a Ca2+-calmodulin kinase, a PDZ, an SH3, and a guanylate kinase domain. CASK localizes at cell–cell adhesion sites, including synaptic junctions, epithelial junctions, and tight junctions, where it is reported to associate with other cell adhesion molecules, such as junctional adhesion molecule (JAM), cadherins, catenins, and syndecan-2. CASK and syndecan-2 expressions are upregulated in colorectal cancer and their upregulation significantly correlates with advanced tumor stage, lymphatic invasion, lymph node metastasis, vascular invasion, liver metastasis, and unresectable metastatic CRC.

~115 kDa observed

Epitope: Internal (C-terminal half)

GST-tagged recombinant protein corresponding to an internal fragment within the C-terminal half of human CASK.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CASK in human kidney cortex tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-CASK Antibody, clone 2G5.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CASK.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CASK in 10 µg of rat brain tissue lysate.

Concentration: Please refer to lot specific datasheet.

Replaces: MAB5230

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