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Merck

MABN665

Anti-Synaptotagmin-7 Antibody, clone N275/14

clone N275/14, from mouse

別名:

Synaptotagmin-7, Synaptotagmin VII, SytVII

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
N275/14, monoclonal
Application:
IHC, WB
Citations:
5
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biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

N275/14, monoclonal

species reactivity

mouse, human, rat

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SYT7(9066)

General description

Synaptotagmin-7 is also referred to as Synaptotagmin VII (SytVII) and is a member of the synaptotagmin family of transmembrane transporters. Synaptotagmin-7 is involved in plasma membrane repair, and possibly Ca2+-dependent exocytosis and traffiking of secretory vesicles.
~ 45 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: Cytoplasmic C2A domain
Recombinant protein corresponding to the cytoplasmic C2A domain of mouse Synaptotagmin-7.

Application

Anti-Synaptotagmin-7 Antibody, clone N275/14 is a highly specific mouse monoclonal antibody, that targets Synaptotagmin & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptotagmin-7 in human thalamus tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Biochem/physiol Actions

This antibody recognizes the cytoplasmic C2A domain of Synaptotagmin-7. This antibody does not cross react with Synaptotagmin-6 or other Synaptotagmins (Prof. J. Trimmer, University of California, Davis.).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate
Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synaptotagmin-7 in 10 µg of mouse brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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