MABN713
Anti-CA9 Antibody, clone 2D3
ascites fluid, clone 2D3, from mouse
別名:
Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, Membrane antigen MN, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9
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この商品について
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2D3, monoclonal
Application:
FACS, IHC, WB
Citations:
-
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2D3, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CA9(768)
General description
CA9 (Carbonic anhydrase 9) is a single-pass type I membrane protein belonging to the alpha-carbonic anhydrase family. CA9 may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. CA9 is primarily expressed in carcinoma cell lines and is found on the surface microvilli and in the nucleus.
~50 kDa observed. Uncharacterized bands may appear in some lysate(s).
Immunogen
Purified recombinant fragment of human CA9 expressed in E. Coli.
Application
Anti-CA9 Antibody, clone 2D3 is a highly specific mouse monoclonal antibody, that targets Carbonic Anhydrase & has been tested in western blotting, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CA9 in human stomach and colon tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CA9 in NTERA-2 cells.
Optimal working dilutions must be determined by end user.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CA9 in NTERA-2 cells.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa and A549 cell lysates.
HeLa and A549 cell lysates.
Evaluated by Western Blotting in HeLa and A549 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CA9 in HeLa and A549 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CA9 in HeLa and A549 cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
MABN713:
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