Anti-BIG1 Antibody, clone 102

Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (UniProt: Q9Y6D6; also known as BIG1, Brefeldin A-inhibited GEP 1, ADP-ribosylation factor guanine nucleotide-exchange factor 1, p200 ARF guanine nucleotide exchange factor, p200 ARF-GEP1) is encoded by the ARFGEF1 (also known as ARFGEP1, BIG1) gene (Gene ID: 10565) in human. BIG1 is expressed in placenta, lung, heart, brain, kidney and pancreas. BIG1 activates ADP-ribosylation factor (ARF) GTPases and is involved in vesicular trafficking. It promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. BIG1 is required for the maintenance of Golgi structure and this function may be independent of its GEF activity. BIG1 and BIG2 display about 74% homology in their amino acid sequence and about 90% identity in their Sec7 domains. BIG1 is phosphorylated by PKA that reduces its GEF activity. It is localized in the cytoplasm in a multiprotein complex along with BIG2. Its nuclear localization has also been reported and it contains a nuclear localization sequence (aa 711-715). In serum starved cells treated with FK506, BIG1 is essentially completely concentrated in nuclei. BIG1 is shown to be a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity. Here BIG1 and RhoA compete with each other for the binding to myosin IXb, thus resulting in the inhibition of the GAP activity by BIG1. (Ref.: Lin, S et al. (2013). Arterioscler Thromb Vasc Biol. 33(2): e31-e38).

~209 kDa observed; 208.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

GST-tagged recombinat fragment corresponding to 311 amino acids from human Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1).

Detect BIG1 using this mouse monoclonal Anti-BIG1 Antibody, clone 102, Cat. No. MABS1247. It has been tested in Immunocytochemistry and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 0.5 µg/mL of this antibody detected BIG1 in 10 µg of A431 and HEK293 cell lysates.

Immunocytochemistry Analysis: A representative lot detected BIG1 in Immunocytochemistry applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).

Western Blotting Analysis: A representative lot detected BIG1 in Western Blotting applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).

Clone 102 specifically detects BIG1 protein in human and targets an epitope within amino acids 212-522 in the N-terminal region.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected BIG1 in 10 µg of Jurkat cell lysate.

Concentration: Please refer to lot specific datasheet.

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