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Merck

MABS465

Anti-Nampt, clone 14A.5 Antibody

clone 14A5, from rat

別名:

PBEF, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1 homolog, PBEF, Visfatin

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
14A5, monoclonal
Application:
ELISA, IHC, WB, inhibition assay
Citations:
3
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biological source

rat

Quality Segment

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

14A5, monoclonal

species reactivity

human, mouse

technique(s)

ELISA: suitable, immunohistochemistry: suitable, inhibition assay: suitable, western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NAMPT(10135)

General description

Nicotinamide phosphoribosyltransferase (NAMPT) is a member of the NAPRTase family. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. NAMPT is localized in the cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis.
~56 kDa observed

Immunogen

Recombinant protein corresponding to mouse Nampt.

Application

Anti-Nampt antibody, clone 14A.5 is an antibody against Nampt for use in western blotting, IHC, inhibition & ELISA.
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Nampt in Anti-CD3epsilon stimulated mouse spleen cell lysate (Prof. Oberdan Leo, Universite Libre de Bruxelles.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Nampt in mouse spleen tissue lysate (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Inhibition Analysis: A representative lot from an independent laboratory was inhibited by a synthetic peptide corresponding to murine Nampt in total liver extracts (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Nampt in CD11c positive mouse spleen sections (Prof. Oberdan Leo, Universite Libre de Bruxelles.).

ELISA Analysis: A representative lot from an independent laboratory detected recombinant Nampt peptides (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nampt in 10 µg of Raji cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


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保管分類

12 - Non Combustible Liquids

wgk

WGK 2



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