Anti-MAP2K4, clone 5H4 Antibody

Purified recombinant fragment of human MAP2K4 expressed in E. Coli.

~40 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected MAP2K4 in muscle tissue.

Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected MAP2K4 in K562 cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Mouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in HepG2, K562 and HEK293 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K4 in HepG2, K562 and HEK293 cell lysates.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.