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NA57

Anti-DNA-PK (Ab-2) Mouse mAb (18-2)

liquid, clone 18-2, Calbiochem®

別名:

Anti-DNA Dependent Protein Kinase

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この商品について

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
18-2, monoclonal
Species reactivity:
human
Application:
—
Citations:
11
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biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

18-2, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Frozen Sections (1:50-1:100)
Immunoblotting (1:250-1:500)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (10 µL/mg protein lysate)
Neutralization Studies
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... PRKDC(5591)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing the splenocytes with mouse myeloma SP2/0 cells (see application references). Recognizes the ~460 kDa DNA-PK protein.
Recognizes the ~470 kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.
This Anti-DNA-PK (Ab-2) Mouse mAb (18-2) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of DNA-PK (Ab-2).

Immunogen

DNA-PK purified from HeLa cells
Epitope: within amino acids 1-2713 of DNA-PK
Human

Application



Frozen Sections (1:50-1:100)
Immunoblotting (1:250-1:500)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (10 l/mg protein lysate)
Neutralization Studies (see comments)
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Preparation Note

For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Analysis Note

Positive Control
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue

Other Notes

Kharbanda, S., et al. 1997. Nature386, 732.
McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.
Peterson, S.R., et al. 1997. J. Biol. Chem.272, 10227.
Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.
Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.
Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol.8, 153.
Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem.271, 8936.
Connelly, M.A., et al. 1996. Gene175, 271.
Danska, J.S., et al. 1996. Mol. Cell. Biol.16, 5507.
Han, Z., et al. 1996. J. Biol. Chem.271, 25035.
Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.
Song, Q., et al. 1996. EMBO J.15, 3238.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Blunt, T., et al. 1995. Cell80, 813.
Hartley, K.O., et al. 1995. Cell82, 849.
Kirchgessner, C.U., et al. 1995. Science267, 1178.
Lees-Miller, S.P., et al. 1995. Science267, 1183.
Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.
Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.
Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.
This antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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