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NB20

Anti-AnkyrinG Mouse mAb (463)

liquid, ≥95% (SDS-PAGE), clone 463

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この商品について

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
463, monoclonal
Species reactivity:
rat, mouse, human, canine
Application:
—
Citations:
4
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biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

463, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, mouse, human, canine

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Frozen Sections (1-2 µg/mL)
Immunoblotting (2-5 µg/mL)
Immunofluorescence (1-2 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ANK3(288)

General description

Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of AnkyrinG.
Recognizes the ~190 kDa ankyrinG protein in brain extracts. Reacts with all spliced forms of ankyrinG.
  • Antibody Target Gene Symbol: ANK3
  • Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
  • Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
  • Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)
  • Mu Entrez ID: 11735
  • Rat Entrez ID: 361833

    • Immunogen

    Human
    a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG

    Application


    Frozen Sections (1-2 g/ml)
    Immunoblotting (2-5 g/ml)
    Immunofluorescence (1-2 g/ml)

    Packaging

    Please refer to vial label for lot-specific concentration.

    Physical form

    In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

    Analysis Note

    Positive Control
    Mouse or rat brain extract or unmyelinated axons

    Other Notes

    AnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
    Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.
    Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
    Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
    Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
    Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
    Otto, E., et al. 1991. J. Cell Biol.114, 241.
    Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    Disclaimer

    Toxicity: Standard Handling (A)

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    保管分類

    10 - Combustible liquids

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