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Merck

PC176

Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb

liquid, Calbiochem®

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この商品について

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, sheep, rat
Application:
Citations:
5
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biological source

rabbit

Quality Segment

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, sheep, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Frozen Sections (1:300-1:500)
Immunoblotting (1:500-1:1000)
Free Floating Sections
Paraffin Sections (s; heat pre-treatment required))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Htr2A(29595)

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
This Anti-Serotonin 2A Receptor Rabbit pAb is validated for use in Frozen Sections, WB, Free Floating Sections, Paraffin Sections for the detection of Serotonin 2A Receptor.

Immunogen

Rat
a synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor

Application


Frozen Sections (1:300-1:500)
Immunoblotting (1:500-1:1000)
Free Floating Sections (see application references)
Paraffin Sections (see application references; heat pre-treatment required)

Physical form

In 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Antibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
Leonard, B.E. 1996. Psychother. Psychosom.65, 66.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol.8, 175.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


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保管分類

12 - Non Combustible Liquids

wgk

WGK 2



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