PEZGS0416
Millicell® EZスライド
4-well glass chamber slide, 1.7 cm2/well, 16/pk, sterile
別名:
4ウェルガラススライド, チャンパースライド
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この商品について
Quality Level
material
polypropylene wells, polystyrene lid, soda-lime glass slide
sterility
ethylene oxide treated, sterile
feature
Raised corner ridges on the lid facilitate stacking of multiple units
packaging
box of 16 (4 trays, 4 units/tray)
manufacturer/tradename
Millicell®
technique(s)
cell based assay: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable
L × W × H
75 mm × 25 mm × 1 mm, slide only, 77.8 mm × 33.8 mm × 13.9, excluding lid
L × W
77.8 mm × 33.8 mm × 16.2 mm, including lid
size
4 wells
well surface area
1.7 cm2
well volume
500-1700 μL
shipped in
ambient
storage temp.
10-30°C
General description
Preparation Note
- 推奨プロトコールに従って細胞を培養、固定、染色します。
- 顕微鏡スライドを利用するときは、Millicell® EZ SLIDEを平らな面に置き、親指で4つのタブを押し下げてカバーを取り外します。スライドを押し下げて、ウェルを上に持ち上げて取り外します。スライドをホルダーから取り外します。
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試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.
High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
関連コンテンツ
Expression of genetically-encoded fluorescently-tagged proteins has widely been employed for real-time visualization of cellular behavior and trafficking. Prepackaged, ready-to-use, high-titer lentiviral particles (which we have termed “lentiviral biosensors”) encoding GFP- or RFP-tagged proteins are a convenient, robust solution for fluorescent imaging of transduced cells. Compared to other nonviral transfection methods, lentiviral transduction, in many cases, offers higher transfection efficiency and more homogeneous protein expression, particularly for traditionally hard-to-transfect primary cell types. Lentiviral biosensors are ideal for use with fixed and live cell fluorescent microscopy, and are non-disruptive towards cellular function. GFP- or RFP-protein localization matches well with antibody-based immunostaining and demonstrates altered patterns of expression upon treatment with modulators of cell function and phenotype. Lentiviral biosensors provide a broadly effective, convenient method for visualization of cell behavior under a variety of physiological and pathological treatment conditions, in both endpoint and real-time imaging modalities. In this study, we focus on lentiviral biosensors containing GFP-LC3 and RFP-LC3 for the study of autophagosome formation.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| PEZGS0416 | 04053252699948 |