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About This Item
UNSPSC Code:
41106514
NACRES:
NA.76
eCl@ss:
32011203
Quality Segment
description
Cell line type: Embryonic Stem Cells, Mouse Embryonic Stem Cells
technique(s)
cell culture | stem cell: suitable
General description
Embryonic Stem Cell line derived from C3H/HeN blastocysts. C3H/HeN is a widely used inbred strain, commonly used for pharmacological testing. F1 mice produced from C57BL/6 and C3H parental strains are commonly used in toxicological testing. C3H mice show a high frequency of mammary tumors when reared in non-SPF conditions due to viral transmission from mother to offspring during rearing. The C3H/HeN strain carries the rd1 (retinal degeneration) mutation. They carry the H2k haplotype. Additional phenotypic information is available at Mouse Genome Informatics website (http://www.informatics.jax.org/external/festing/mouse/docs/C3H.shtml). The C3H ES line was genome verified by determining the alleles at 29 SNPs that are informative across a large number of inbred mouse strains.
STRAIN: C3H/HeN
GENDER: Male
KARYOTYPE: 40XY
STRAIN: C3H/HeN
GENDER: Male
KARYOTYPE: 40XY
Packaging
2.5 x 106 cells per vial, passage 9
Physical form
Cells are supplied frozen in basal medium supplemented with 50% FCS and 10% DMSO
Preparation Note
Place vials in liquid nitrogen immediately upon receipt until it is convenient to proceed to subculture.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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