CNS-1 Rat Glioma Cell Line

Each vial contains ≥1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Rat/Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.Cells are verified to be of rat origin and negative for inter-species contamination from mouse, human, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic ServicesCells are negative for mycoplasma contamination.

Glioma is one of the most common brain tumors and is highly aggressive and resistant to standard treatments. Immunotherapy is one of the more promising treatment options since the discovery of lymphatics in the central nervous system allows for penetration of the blood-brain barrier (1). Cellular models that both recapitulate in vivo characteristics of gliomas and are amenable to immunotherapeutic modalities are thus of great value in advancing understanding of gliomas and their potential treatment.The CNS-1 rat glioma cell line is a well-established model that closely mimics the invasive behavior and growth characteristics of human gliomas (2). CNS-1 cells are derived from an N-nitroso-N-methylurea-induced intracranial tumor of a male Lewis rat and are histocompatible with this inbred rat strain, facilitating a range of in vivo studies. Tumors derived from injection of CNS-1 cells display diffuse infiltrative patterns similar to human glioblastoma multiforme (2,3). CNS-1 cells are positive for glial fibrillary acid protein (GFAP), retinoic acid receptor α, and neural cell adhesion molecule NCAM-140 (2,4). CNS-1 cells exhibit multiple chromosome loss and rearrangements reflecting genetic instability common in many human gliomas (2). CNS-1 rat glioma cells are a highly relevant model with wide applications for glioma immunotherapies.CNS-1 cell line originated from an N-nitroso-N-methylurea-induced intracranial tumor of a male Lewis rat (1). 1. Cancer Lett 2020, 476: 1-12.2. J Neurooncol 1994, 22(3): 191-200.3. J Neurooncol 2009, 94(3): 299-312.4. Cancer Res 1998, 58(9): 2020-2028.

CNS-1 rat glioma cells are a highly relevant model with wide applications for glioma immunotherapies.​

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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