B8931
Magenta-Gal
reagent for selection of recombinant bacterial clones
別名:
Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside
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この商品について
実験式（ヒル表記法）:
C14H15BrClNO6
CAS番号:
分子量:
408.63
NACRES:
NA.85
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
grade
Molecular Biology
sterility
non-sterile
product line
BioReagent
assay
≥98.0% (HPLC)
form
powder
solubility
methanol: soluble
suitability
suitable for substrate for β-galactosidase
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1
InChI key
CHRVKCMQIZYLNM-HTOAHKCRSA-N
General description
Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Application
Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.
Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.
Biochem/physiol Actions
When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos
In Situ Hybridization Methods, 151-167 (2015)
George Chenaux et al.
The European journal of neuroscience, 34(10), 1620-1633 (2011-11-23)
EphB receptor tyrosine kinases direct axonal pathfinding through interactions with ephrin-B proteins following axon-cell contact. As EphB:ephrin-B binding leads to bidirectional signals, the contributions of signaling into the Eph-expressing cell (forward signaling) or the ephrin-expressing cell (reverse signaling) cannot be
Susan C McCutcheon et al.
BMC biology, 8, 89-89 (2010-06-24)
Reporter genes are widely used in biology and only a limited number are available. We present a new reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum