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SCT150 Firefly Luciferase HTS Assay

Glow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours

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SCT150
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      概要

      Replacement Information
      Description
      Catalogue NumberSCT150
      DescriptionFirefly Luciferase HTS Assay
      OverviewFirefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Firefly luciferase is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin, producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules.

      However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. The Firefly Luciferase HTS Assay is designed for is a proprietary mixture of substances that modify the enzymatic reaction to produce a long-lasting signal (steady-glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit for the quantification of firefly luciferase expression in mammalian cells with signal half-life of about 3 hours (Figure 2). Glow-type luciferase assays have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.
      Alternate Names
      • Firefly Luciferase Assay
      • Bioluminescent Assay
      References
      Product Information
      Components
      • 1) Assay Buffer (CS224524): 1X100 ml
      • 2) D-Luciferin (CS224523): 1 X 25 mg
      Detection methodChemiluminescence
      PresentationLiquid
      Applications
      ApplicationGlow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours
      Key Applications
      • Cell Based Assays
      Application NotesFirefly Luciferase Assay
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore Firefly Luciferase HTS Assay at -80°C. Kit components are stable for at least six months from date of receipt when stored as recommended. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      SCT150 04054839566028