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Merck

MABE967

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1

clone 11E10.1, from mouse

동의어(들):

DNA polymerase delta catalytic subunit, DNA polymerase delta p125, DNA polymerase subunit delta p125

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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제품 이름

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1, clone 11E10.1, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

11E10.1, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001243778

UniProt accession no.

P28340

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... POLD1(5424)

Analysis Note

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA polymerase delta p125 in 10 µg of Raji cell lysate.

Application

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1 is an antibody against DNA polymerase delta p125 Antibody for use in Western Blotting.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DNA polymerase delta p125 in 10 µg of Ramos cell lysate.

General description

DNA polymerase delta catalytic subunit (EC 2.7.7.7; UniProt P28340; also known as CDC2 homolog, DNA polymerase subunit delta p125) is encoded by the POLD1 (also known as CDC2, MDPL, POLD, CRCS10) gene (Gene ID 5424) in human. DNA replication in eukaryotes involves multiple enzymes including the B family polymerases Pol α, Pol δ, and Pol ɛ. While Pol α initiates DNA synthesis, Pol δ and Pol ɛ replace Pol α after primer extension and perform the bulk of DNA replication. Pol δ and Pol ɛ are heterotetramers in higher eukaryotes comprised of POLD1 and POLE as the respective catalytic subunit, as well as accessory subunits (POLD2/3/4 and POLE2/3/4) that interact with cofactors such as Proliferating Cell Nuclear Antigen (PCNA). Both POLD1 and POLE contain an exonuclease domain (a.a. 268–471 of POLE and 304–517 of POLD1) that proof-reading capability during DNA replication. In addition to DNA synthesis, Pol ɛ and Pol δ are believed to participate in chromosomal DNA repairs, including nucleotide excision repair (NER), ismatch repair (MMR) and repair of double strand breaks (DSBR). Germline exonuclease domain mutations (EDMs) in POLD1 and POLE cause polymerase proofreading-associated polyposis (PPAP). Tumors with EDMs exhibits dramatically increased base substitutions.
~125 kDa observed.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of human DNA polymerase delta p125.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

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관련 콘텐츠

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Post-Translational Modifications

국제 무역 품목 번호

SKUGTIN
MABE96704055977294743

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