제품 이름
Anti-GABA Rabbit pAb, lyophilized, Calbiochem®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Frozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; ))
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
rat ... Gabra1(29705)
Application
Frozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Anti-GABA, rabbit polyclonal, recognizes GABA in rat thalamus and cerebellum. It is validated for use in immunohistochemistry on frozen sections.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes GABA.
Recognizes GABA in rat thalamus and cerebellum.
Immunogen
GABA
Rat
Analysis Note
Positive Control
Rat thalamus or cerebellum
Rat thalamus or cerebellum
Other Notes
Antibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Physical form
Undiluted serum.
Preparation Note
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| PC213L-100ULCN | 04055977224665 |
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