제품 이름
Anti-Tie2 Mouse mAb (Ab33), liquid, clone Ab33, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
Ab33, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, porcine, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections
Immunoblotting (0.2-1 µg/mL, chemiluminescence)
Immunoprecipitation
Paraffin Sections (not recommended))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TEK(7010)
Application
Frozen Sections (see comments)
Immunoblotting (0.2-1 µg/ml, chemiluminescence)
Immunoprecipitation (see comments)
Paraffin Sections (not recommended)
Immunoblotting (0.2-1 µg/ml, chemiluminescence)
Immunoprecipitation (see comments)
Paraffin Sections (not recommended)
Disclaimer
Toxicity: Standard Handling (A)
General description
Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
Recognizes the ~140-45 kDa Tie2 protein.
This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.
Immunogen
Human
a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag sequence
Other Notes
For frozen sections, tissues were treated with Triton™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Nattawat Klomjit et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 37(10), 1844-1856 (2022-04-23)
Renal artery stenosis (RAS) is an important cause of chronic kidney disease and secondary hypertension. In animal models, renal ischemia leads to downregulation of growth factor expression and loss of intrarenal microcirculation. However, little is known about the sequelae of
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