208771 Calpain Substrate III, Fluorogenic - Calbiochem

208771
가격을 검색할 수 없습니다
Minimum Quantity needs to be mulitiple of
가격 문의 추가 정보
()이 할인됨
 
가격 문의
재고 없음재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service
      가격 및 재고 조회

      개요

      Replacement Information

      가격 및 재고여부

      카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
      208771-1MGCN
      재고 정보 검색...
      재고 없음재고 없음
      예상 출고 가능일 
      단종품
      제한된 수량 가능
      재고여부 확인
        Remaining: will advise
          Remaining: will advise
          추천사항
          고객 서비스로 문의
          Contact Customer Service

          Plastic ampoule 1 mg
          가격 검색...
          가격을 검색할 수 없습니다
          Minimum Quantity needs to be mulitiple of
          가격 문의 추가 정보
          ()이 할인됨
           
          가격 문의
          Description
          OverviewA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
          Catalogue Number208771
          Brand Family Calbiochem®
          Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
          References
          ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
          Product Information
          ATP CompetitiveN
          FormRed lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₈₀H₁₁₄N₂₀O₁₈S
          Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
          ReversibleN
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetSubstrate sequence that is optimized for calpain-1 and -2
          Primary Target IC<sub>50</sub>kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Calpain Substrate III, Fluorogenic - Calbiochem MSDS

          타이틀

          물질안전보건자료(MSDS) 

          Calpain Substrate III, Fluorogenic - Calbiochem Certificates of Analysis

          TitleLot Number
          208771

          References

          참고문헌 보기
          Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-May-2008 RFH
          Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
          DescriptionA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with higher efficiency (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively). It is kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in intact Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
          FormRed lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
          Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
          Purity≥98% by HPLC
          SolubilityDMSO (5 mg/ml) or H₂O (1 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Irritant
          ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.