species reactivity
vertebrates
packaging
pkg of 1 kit
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
shipped in
dry ice
storage temp.
2-8°C
Quality Level
General description
The CHEMICON in vitro angiogenesis assay kit provides a convenient system for evaluation of tube formation by endothelial cells in a convenient 96-well format. When cultured on ECMatrix, a solid gel of basement proteins prepared from the Engelbreth Holm-Swarm (EHS) mouse tumor, these endothelial cells rapidly align and form hollow tube-like structures. Tube formation is a multi-step process involving cell adhesion, migration, differentiation, and growth.
ECMatrix is optimized for maximal tube-formation. For assaying inhibitors or stimulators of tube formation, simply premix the endothelial cell suspension with different concentrations of the inhibitor or stimulator to be tested, before adding the cells to the top of the ECMatrix.
Application
- human umbilical vein cells (HUVEC)
- primary human retinal microvascular cells
- platelet derived growth factor receptor-β (PDGFRβ)/PAE cells
- bovine capillary endothelial (BCE) cells
Preparation Note
Other Notes
- ECMatrixTM Gel Solution: (Part No. 90060) Five 1 mL bottles.
- ECMatrixTM Diluent Buffer, 10X: (Part No. 90061) One 1 mL bottle.
Legal Information
Disclaimer
저장 등급
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
관련 콘텐츠
"The successful, reliable culture of epithelial cells is critical for many areas of research, including dermatology, respiratory research, and cancer research. Because the breakdown of control mechanisms in epithelial cells is a frequent contributor to cancer progression and metastasis, epithelial cell culture is particularly important for cancer research. EpiGRO™ media formulations are optimized to provide better viability, proliferation rates, morphology and culture stability than other commercially available options. The media are provided in unique, light-blocking, temperature-monitored packaging to ensure stability and protect the media from damage by light, contamination, and excessive heat. The media do not require or contain any antimicrobials or phenol red. These components can cause cell stress and influence experimental results by masking the true performance or health of the cell culture. Phenol red acts like an estrogen and may stimulate growth independently of experimental variables. "
"Recognizing both the tremendous opportunities and the challenges facing cancer research, we are dedicated to developing and refining tools and technologies for the study of cancer. With our comprehensive portfolio, including the Upstate®, Chemicon®, and Calbiochem® brands of reagents and antibodies, researchers can count on dependable, high quality solutions for analyzing all the hallmarks of cancer."
Key Steps in Tumor Metastasis
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| ECM625 | 04053252476556 |
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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