modified Tryptic Soy Agar- Dehydrated Culture Media

NutriSelect^® prime Tryptic soy agar with polysorbate 80 and lecithin acc. EP, USP, JP, EN 17141, and ISO 21149 are used for environmental monitoring and determining the efficiency of containers and surfaces. The combination of the two peptones, the enzymatic digest of casein and soybean, provides high nutrition by supplying organic nitrogen, amino acids, and longer-chained peptides. Sodium chloride in this complex medium maintains the osmotic equilibrium, and agar-agar is the solidifying agent. This modification of Tryptic Soy Agar contains lecithin and polysorbate 80 (also known as Tween^® 80) to inactivate residual disinfectants when sampling. Lecithin also neutralizes quaternary ammonium compounds (QACs), para-hydroxybenzoate (parabens), and bis-biguanides. Likewise, polysorbate 80 neutralizes QACs, iodine, parabens, phenols, hexachlorophene, and formalin. Tryptic Soy Agar (TSA) with Lecithin and Polysorbate 80 can be used to prepare plates for different usage (such as contact plates for surface-sampling, plates for surface-plating and air-sampling techniques, membrane filtration and pour-plating technique).

NutriSelect^® prime Tryptic soy agar with polysorbate 80 and lecithin are suitable for the isolation and cultivation of a wide range of microorganisms surviving after treatment of surfaces and materials with antiseptics and for the enumeration and detection of aerobic mesophilic bacteria (Microbial Limit Test) from cosmetic products containing preservatives.

Appearance (clearness): clear
Appearance (color): yellowish-brown
pH-value (25 °C): 7.1 - 7.5
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 10 - 100
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): 10 - 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 10 - 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 21059): 10 - 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): 10 - 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): 10 - 100
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): 10 - 100
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Streptococcus pyogenes ATCC 21059):
Colony count (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)):
Colony count (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)):
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 70 %
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Streptococcus pyogenes ATCC 21059): ≥ 70 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): ≥ 70 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): ≥ 70 %
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Test for neutralizing capacity (Staphylococcus aureus ATCC 6538 (WDCM 00032)): passes test
Test for neutralizing capacity (Candida albicans ATCC 10231 (WDCM 00054)): passes test
Incubation: 24 hrs.; 30-35 °C; aerobic;
C.albicans and A. brasiliensis up to 5 days.
Reference: blood-agar; for C.albicans and A. brasiliensis sabouraud 2% dextrose-agar; for the test for neutralizing capacity tryptic soy agar.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^®culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.