제품 이름
Purospher® STAR NH2 (5 µm), L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC column
material
stainless steel column
agency
suitable for USP L8
product line
Purospher®
feature
endcapped: no
packaging
1 ea of
extent of labeling
3.5% Carbon loading
parameter
400 bar pressure, 65 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
25 cm × 4.6 mm
surface area
330 m2/g
matrix
fully porous particle
matrix active group
NH2, NH2 bonding phase
particle size
5 μm
pore size
120 Å pore size
operating pH range
1.5-10.5
separation technique
hydrophilic interaction (HILIC), normal phase, reversed phase, supercritical fluid (SFC)
storage temp.
no temp limit
General description
Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm Hibar® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART™ 4-4 part number 1.50267.0001 [https://www.sigmaaldrich.com/US/en/product/mm/150267]. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART™ cartridges, part number 1.51487.0001 can be used. The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide
Application
Intended for the separation of alcohols, carboxylic acids, fat-soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water-soluble vitamins.
Features and Benefits
- Reproducible results from run-to-run and batch-to-batch
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
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