HPLC 컬럼

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• Flyer: Ascentis^® Express 160Å ZIC^®-cHILIC Columns

  The Ascentis^® Express ZIC^®-cHILIC column is designed for the effective retention and separation of polar and hydrophilic compounds, delivering robust chromatography with high selectivity and reproducibility.

• Flyer: Ascentis^® Express 120 Å C18 pH+ U/HPLC Columns

  The Ascentis^® Express 120 Å C18 pH+ U/HPLC column is built on the reliable Fused-Core^® particle technology, enhancing speed and efficiency. It features surface-modified organo-silane technology that provides alkaline resistance, ensuring excellent stability in high pH environments.

• /deepweb/assets/sigmaaldrich/marketing/global/documents/660/486/trypsin-cleaved-proteins-capillary-columns-wp14380en-ms.pdf

  This study employs a Two-Dimensional Liquid Chromatography (2D-LC) approach, utilizing both Reversed-Phase Liquid Chromatography (RPLC) and Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC^®-HILIC), combined with Time-of-Flight Mass Spectrometry (TOF-MS) detection, to analyze trypsin-cleaved proteins including Bovine Serum Albumin (BSA), cytochrome C, and ovalbumin.

• Flyer: Purospher^® STAR RP-18 endcapped, 2 µm Capillary Columns

  Best in Class C18 phase for excellent peak symmetry, performance and pH stability.

• Flyer: Ascentis^® Express PCS Phenyl-Hexyl, 2.7 µm U/HPLC Columns

  The Ascentis^® Express 90 Å PCS Phenyl-Hexyl 2.7 µm U/HPLC column offers an alternative selectivity in comparison to C18 columns including PCS-C18 providing benefits for the separation of basic aromatic compounds with improved peak shape.

• Flyer: Ascentis^®Express PCS-C18, 2.7 µm U/HPLC Columns

  The Ascentis^® Express PCS-C18 columns are designed for effective separation of basic, acidic, or neutral compounds using low ionic strength (formic acid) mobile phase conditions.

• Flyer: SeQuant^® ZIC^®-HILIC and ZIC^®-cHILIC Capillary Columns

  SeQuant^® ZIC^®-HILIC and ZIC^®-cHILIC columns are high-performance tools designed for separating polar hydrophilic compounds under hydrophilic interaction liquid chromatography (HILIC) conditions.

• Brochure: HPLC Column Selection Guide

  The comprehensive Supelco^® portfolio of analytical solutions to meet your U/HPLC and LC-MS needs.

• Brochure: HPLC Method Development Guide

  High performance liquid chromatography (HPLC) is a technique for separating analytes dissolved in a liquid, mobile phase by using their specific interaction with a stationary phase (chromatography column).

• Brochure: Ascentis^® Express Columns

  Based on Fused-Core^® particle technology, Ascentis^® Express columns provide a exceptional advancements in HPLC column performance and the benefits of high sample throughput at maximum resolution.

• Brochure: BIOshell™ Columns

  The BIOshell™ line of superficially porous particle (SPP) packed columns has been developed to aid research into understanding the subtleties of the molecule that is being developed.

• Brochure: Chromolith^® HPLC Columns

  To truly accelerate chromatographic separations, there is no better choice than Chromolith® HPLC columns. Due to their revolutionary, monolithic technology, Chromolith® columns provide excellent and rapid separations with extremely high robustness and matrix tolerance compared to particulate columns.

• Brochure: Purospher STAR Columns

  The success of any method depends on the quality of the stationary phase. Precise, long-term reproducibility is a key factor in achieving reliable results. The base silica of Purospher™ STAR columns is 99.999% pure.

• Brochure: Chiral HPLC Columns

  Columns for versatile and robust Chiral HPLC and LC-MS separations.

• Brochure: HPLC Accessories

  The use of HPLC systems to achieve faster analyses and increased resolution pushes systems toward higher operating pressures. For instance, faster flow rates, longer column lengths, and reduced particle sizes gain a performance advantage in HPLC, but at a cost of increased operating pressures.

• Brochure: Discovery Bio Columns

  Many of the challenges facing researchers in the proteomics and biopharmaceutical fields are related to the need to obtain as much information as possible on very limited samples. We designed Discovery^® BIO Wide Pore reversed-phase HPLC columns to address these challenges.

• Brochure: LC-MS Resource Guide

  In this guide, we provide a premier selection of proven Supelco^® tools and consumables that meet the requirements of scientists who primarily use the LC-MS technique for analysis of drugs, biomolecules separation and analytical assays.

• Brochure: LC-MS Contaminants

  In this technical bulletin, you will learn some tips on identifying LC-MS contaminants and avoiding contamination. Our 110 years of separation expertise, combined with our precision-manufactured products, give you the greatest chance of obtaining reproducible, clean data.

• Application Note: Supelco^® HPLC Products: The Best Choice for Any LC Instrument

  Since the 1970’s, Supelco^® has been a trusted name in high performance liquid chromatography (HPLC) consumables. From a diverse array of columns packed with different stationary phases to a complete portfolio of HPLC accessories, the Supelco^® portfolio of HPLC consumables caters to the needs of all analysts.

• Application Guide: Drug of Abuse

  Application compilation with focus on the LC-MS analysis of drugs of abuse in biological samples.

• HPLC Method Transfer Calculator

  Easily transfer a method between HPLC columns, (scale between microbore through preparative range), based on the 2 column dimensions and current method conditions.

• Wall Chart: HPLC Packings for USP Compendial Methods

  The official pharmaceutical analysis monographs in the United States Pharmacopeia (USP) detail the methods used by pharmaceutical manufacturers for quality control of bulk drug substances and dosage form preparations.

• Application Guide: Ascentis^® Express and BIOshell™ Column Cleaning Procedures and Best Practices

  To maximize the lifetime of an HPLC column and ensure its top performance, it is necessary to properly maintain the column. Many different factors can contribute to loss of resolution or sensitivity, but often times the following factors lead to the vast majority of column performance issues.

• Flyer: Supel™ Carbon LC Capillary UHPLC Columns

  Superior separation of challenging compounds at the capillary scale.

• White Paper: Retention Behavior of Mono- and Disaccharides on a Supel™ Carbon LC column

  PGC LC-MS-based profiling of mono- and disaccharides.